General description
The protein HEC1 (highly expressed in cancer 1) is an essential component of the vital NDC80 kinetochore-associated protein complex. The NDC80 complex is required for chromosome segregation and spindle checkpoint activity. HEC1 is essential for proper kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. HEC1 is expressed by all dividing cells and is particularly high in cancers. HEC1 localizes to kinetochores from late prophase to anaphase, specifically at the outer plate of the kinetochore. HEC1 is normally phosphorylated during its active phase at multiple sites and the phosphorylation appears to tune kinetochore-microtubule binding affinity during mitosis. Moreover, recent research using small molecule inhibitors to HEC1′s kinase, Nek2, demonstrate that phosphorylation is required for proper HEC1 function.
~78 kDa observed. Uncharacterized band(s) may appear in some lysates.
Immunogen
Epitope: Nuclear localization region
KLH-conjugated linear peptide corresponding to the Nuclear localization region of human Hec1.
Application
Anti-Hec1 Antibody is an antibody against Hec1 Antibody for use in Western Blotting, Immunocytochemistry.
Immunocytochemistry Analysis: 1.0 µg/mL from a representative lot detected Hec1 in HeLa cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in SK-OV-3 cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected Hec1 in 10 µg of SK-OV-3 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology), mouse (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| NCBI accession no. | NP_006092 |
| UniProt accession no. | O14777 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... NDC80(10403) |
