General description
Purified mouse monoclonal antibody. Recognizes the ~40 kDa PDK2 protein.
Recognizes the ~40 kDa PDK2 protein in U-2 OS cells.
This Anti-PDK2 Mouse mAb (2G1) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of PDK2.
Immunogen
A recombinant polypeptide corresponding to amino acids of 187-276 human PDK2, expressed as a GST fusion protein
Application
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (0.8 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
Positive Control
U-2 OS cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 2G1, monoclonal |
| form | liquid |
| does not contain | preservative |
| species reactivity | human |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (ELISA Immunoblotting (1-5 µg/mL) Paraffin Sections (0.8 µg/mL)) |
| isotype | IgG2a |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... PDK2(5164) |
