General description
Holliday junction recognition protein (HJURP) is also known as 14-3-3-associated AKT substrate (FAKTS), Fetal liver-expressing gene 1 protein (FLEG1) and Up-regulated in lung cancer 9 (URLC9). HJURP is a centromeric protein that acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. HJURP is highly expressed in the thymus, testis, and in many lung cancer samples with lower levels found in placenta, small intestine, liver, skeletal muscle, and colon.
~90 kDa observed
Immunogen
Recombinant protein corresponding to human HJURP.
Application
Detect Holliday junction recognition protein using this mouse monoclonal antibody, Anti-HJURP Antibody, clone 144K/B1 validated for use in western blotting & IP.
Immunoprecipitation Analysis: A representative lot immunoprecipitated HJURP in HeLa nuclear extract (Ana Losada, CNIO, Madrid, Spain).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot immmunoprecipitated HJURP in HEK293 cell lysate (Ana Losada, CNIO, Madrid, Spain).
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected HJURP in 10 µg of HEK293 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 144K/B1, monoclonal |
| species reactivity | human |
| technique(s) | ChIP: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_060880.3 |
| UniProt accession no. | Q8NCD3 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... HJURP(55355) |
