General description
Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
Immunogen
Epitope: Recognizes an epitope on the outside of the regulatory N-terminus of tyrosine hydroxylase.
Tyrosine Hydroxylase purified from PC12 cells.
Application
Anti-Tyrosine Hydroxylase Antibody, prediluted, clone LNC1 detects level of Tyrosine Hydroxylase & has been published & validated for use in IH(P).
Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.
Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.
Incubation: 30 minutes with IHC Select
Tyrosine Hydroxylase has been prediluted for use as the primary antibody with Chemicon′s IHC Select
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Biochem/physiol Actions
Tyrosine Hydroxylase. Recognizes a protein of approximately 59-63 kDa by Western blot. Does not react with the following on Western Blots: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase, phenethanolamine-N-methyl transferase (PNMT).
Physical form
Liquid diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% preservative.
Format: Purified
Preparation Note
Maintain at 2-8°C. Refer to vial for expiration dating.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | LNC1, monoclonal |
| species reactivity | monkey, lizard, mouse, human, zebrafish, vole, frog, rat, chicken |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunohistochemistry: suitable (paraffin) |
| isotype | IgG1κ |
| NCBI accession no. | NM_000360.3,NM_199292.2,NM_199293.2 |
| UniProt accession no. | P07101 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... TH(7054) rat ... Th(25085) |
