General description
58 kDa
Protein phosphatase 5 (PP5) is a novel human protein serine/threonine phosphatase of molecular weight of 58 kDa. It is made up of a C-terminal phosphatase catalytic domain and an N-terminal domain, which has four repeats of 34 amino acids, three of which are tandemly arranged. The phosphatase domain contains all of the invariant motifs of the PP1/PP2A/PP2B gene family. However, it is not closely related to any other known member of this family of phosphatases. Thus it makes up a new subfamily of phosphatases. PP5 expressed in bacteria has been shown to dephosphorylate serine residues in proteins and is more sensitive than PP1 to the tumor promoter okadaic acid. PP5 appears to be localized to the nucleus where it is thought to play a roll in the regulation of RNA biogenesis and mitosis as part of a signaling pathway regulating cell cycle progression.
Immunogen
Synthetic peptide derived from the human protein phosphatase 5 protein.
Application
Detect PP5 using this Anti-PP5 Antibody validated for use in WB, IH(P).
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining of PP5 Polyclonal Antibody: Human Testis
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases
Biochem/physiol Actions
Recognizes Protein Phosphatase 5 (PP5).
Physical form
Format: Purified
Purified rabbit polyclonal IgG in phosphate buffered saline with 0.08% sodium azide
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Jurkat Cell Lysate, A549 and MCF-7 cell lysates.
Routinely evaluated by Western Blot on Jurkat lysates.
Western Blot Analysis: 1:500 dilution of this lot detected PP-5 on 10 μg of Jurkat lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB4092
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | mouse, human |
| manufacturer/tradename | Upstate® |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NM_006247.2 |
| UniProt accession no. | P53041 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PPP5C(5536) |
