General description
S-100 protein is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. The protein exists in both heterodimeric and homodimeric forms. The two subunits of S-100 protein are the products of separate genes that are differentially expressed by various cells. The beta subunit is present in all S-100 positive cells and tumors. In contrast, the alpha subunit is detectable only in heart, skeletal muscle and brain. In addition to its calcium binding properties, S-100 protein has a high-affinity binding site for zinc and is involved in the regulation of protein phosphorylation in the brain. The protein apparently plays a role in cell differentiation and growth, cytoskeletal structure and function, and has been implicated in neuropathological diseases.
~10 kDa observed
Immunogen
Epitope: Near N-Terminus
KLH-conjugated linear peptide corresponding to human S100B near the N-Terminus.
Application
Detect S100 using this rabbit polyclonal antibody, Anti-S100B Antibody validated for use in western blotting & IHC (Paraffin).
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected S100B in human thalamus and human cerebellum sections.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in A375 cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected S100B in 10 µg of A375 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1054
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100,300 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | mouse (based on 100% sequence homology), rat (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), bovine (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| web metadata keyword.default | S100B antibody ab115803,S100B antibody for immunohistochemistry,S100B antibody for tumor identification,S100B antibody suitable for human samples,S100B antibody western blot,S100B beta subunit antibody,S100B protein detection antibody,anti-S100B antibody Sigma-Aldrich,anti-S100B antibody for paraffin sections,anti-S100B antibody,custom S100B antibody production,high purity anti-S100B antibody,purified S100B antibody for research,recombinant S100B antibody for diagnostics |
| NCBI accession no. | NP_006263 |
| UniProt accession no. | P04271 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | rat ... S100B(25742) |
