General description
Axin-2, or Axis inhibition protein 2, is an inhibitor of the Wnt signaling pathway that down-regulates beta-catenin and APC through phosphorylation. Axin-2 abnormalities are linked to defective mismatch repair and have been found to be involved in colorectal cancer (CRC) and oligodontia-colorectal cancer syndrome (ODCRCS). Axin-2 can be found in brain and lymphoblast tissues.
~ 95 kDa observed
Immunogen
Linear peptide corresponding to Human Axin 2.
Application
Immunocytochemistry Analysis: 20 μg/mL of this antibody from a representative lot detected Axin2 in HeLa, HEK293 and NIH-3T3 cells.
Western Blotting Analysis: A representative lot of this antibody detected Axin2 in SW 480 cell lines
Research Category
Signaling
Apoptosis & Cancer
Research Sub Category
General Post-translation Modification
This Anti-Axin2, clone cond4-1D5 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Axin2.
Biochem/physiol Actions
This monoclonal antibody recognizes both endogeneous and overexpressed Axin2 and does not cross-react with Axin1
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HCT116 cell lysate.
Western Blotting Analysis: 1 μg/mL of this antibody detected Axin2 in 10 µg of HCT116 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | cond4-1D5, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_004646 |
| UniProt accession no. | Q9Y2T1 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... AXIN2(8313) |
