General description
C-C chemokine receptor type 8 (UniProt: P56484; also known as C-C CKR-8; CC-CKR-8; CCR-8, CDw198) is encoded by the Ccr8 (also known as Cmkbr8, Ter1) gene (Gene ID: 12776) in murine species. CCR8, a member of the G-protein coupled receptor 1 family, is a multi-pass membrane protein with four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. Its expression is observed in thymus and its higher expression is observed in Treg and T helper 2 (TH2) cells. It is recruited to the inflammation site and has been implicated in allergy and asthma. It serves as a receptor for CCL1/SCY1 chemokine. In human and in murine models CCR8+ Tregs account for 30 to 80% of tumor Tregs in various cancers and only less than 10% in other tissues. However, most tumor-infiltrating conventional T cells are reported to be CCR8 negative. Disruption of CCR8 function is reported to result in reduced accumulation of Treg cells within tumors and disrupts their immunosuppressive function. (Ref.: Kidani, Y., et al. (2022). Proc. Natl. Acad. Sci. USA. 119(7); e2114282119; Tanaka, T., et al. (2021). Monoclonal. Antib. Immunodiagn. Immunother. 40(2); 65-70; Zingoni, A., et al. (1998). J. Immunol. 161(2); 547-551).
Immunogen
Chinese Hamster Ovary (CHO) cells stably expressing mouse CCR8.
Application
Quality Control Testing
Evaluated by Flow Cytometry in Chinese Hamster ovary (CHO) cells stably expressing murine CCR8.
Flow Cytometry Analysis: 1.0 µg of this antibody detected CCR8 in one million Chinese Hamster ovary CHO) cells stably expressing murine CCR8.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected CCR8 in Immunocytochemistry applications (Saito, M., et al. (2022). Monoclon Antib Immunodiagn Immunother. 41(2):115-119).
Flow Cytometry Analysis: A representative lot detected CCR8 in Flow Cytometry applications (Tanaka, T., et al. (2021). Monoclon Antib Immunodiagn Immunother. 40(2):65-70; Saito, M., et al. (2022). Monoclon Antib Immunodiagn Immunother. 41(2):115-119).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biochem/physiol Actions
Clone C8Mab-2 is a rat monoclonal antibody that detects C-C chemokine receptor type 8 (CCR8). It targets an epitope within the extracellular domain.
Physical form
Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide.
Preparation Note
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rat |
| Quality Segment | 200 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | C8Mab-2, monoclonal |
| species reactivity | mouse |
| packaging | antibody small pack of 100 |
| technique(s) | flow cytometry: suitable,immunocytochemistry: suitable |
| isotype | IgG2b |
| epitope sequence | Extracellular domain |
| Protein ID accession no. | NP_031746 |
| UniProt accession no. | P56484 |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Ccr8(12776) |
