General description
Serine/threonine-protein phosphatase 6 catalytic subunit (UniProt: O00743; also known as EC: 3.1.3.16, PP6C) is encoded by the PPP6C (also known as PPP6) gene (Gene ID: 5537) in human. Protein phosphatase 6 (PP6) is a serine/threonine protein phosphatase that is classified as a type 2A phosphatase family member based on its sequence homology to the catalytic subunit of PP2A. It is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. The PP6 holoenzyme is a heterotrimeric complex of a catalytic subunit, a SAPS domain-containing subunit (PP6R) and an ankyrin repeat-domain containing regulatory subunit (ARS). PP6C is the catalytic subunit of PP6 that has multiple metal binding (manganese) sites and binds 2 manganese ions per subunit. It is ubiquitously expressed in all tissues and highest expression levels are observed in in testis, heart, kidney, brain, stomach, liver and skeletal muscle. PP6C is shown to be upregulated in the glioblastoma and its levels correlate with patient survival time. Silencing of PP6C by small interfering RNA induces sensitivity to IR and delayed release from the G2/M checkpoint. Three isoforms of PP6C have been described that are produced by alternative splicing. (Ref.: Shen, Y et al. (2011). Cell Death and Dis. 2, e241; Douglas, P., et al. (2010). Mol. Cell. Biol. 30(6); 1368-1381).
~35 kDa observed; 35.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Serine/threonine-protein phosphatase 6 catalytic subunit.
Application
Anti-PP6C, clone 7G7, Cat. No. MABE1818, is a highly specific mouse monoclonal antibody that targets Serine/threonine-protein phosphatase 6 catalytic subunit and has been tested for use in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected PP6C in lysate from NIH3T3 mouse fibroblasts ectopically expressing N-terminally tagged human PP6 catalytic subunit. (Courtesy of Dr. Stefan Schuechner and Dr. Egon Ogris, Medical University of Vienna, Austria).
Biochem/physiol Actions
Clone 7G7 specifically detects human Serine/threonine-protein phosphatase 6 catalytic subunit. It targets an epitope within 17 amino acids from the C-terminal region.
Physical form
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in MCF7 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected PP6C in MCF7 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 7G7, monoclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology) |
| packaging | antibody small pack of 25 μg |
| technique(s) | western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_002712 |
| UniProt accession no. | O00743 |
| target post-translational modification | unmodified |
| Gene Information | human ... PPP6C(5537) |
