General description
135 kDa
Immunogen
Peptide containing the sequence KK[pT]FK in which pT corresponds to phosphothreonine at residue 495 of human eNOS/NOS III
Application
This Anti-phospho-eNOS/NOS III (Thr495) Antibody is validated for use in WB for the detection of phospho-eNOS/NOS III (Thr495).
Western Blot Analysis:
A 1:2,000-1:10,000 dilution of this lot detected phosphorylated eNOS/NOS III in fetal bovine heart endothelial cell lysate.
Biochem/physiol Actions
Predicted cross-reactivity with human, rabbit, mouse and rat
Recognizes phosphorylated eNOS/NOS III.
Physical form
Cultured supernatant in 0.05% sodium azide
Analysis Note
Control
Fetal bovine heart endothelial cell lysate
routinely evaluated by immunoblot on fetal bovine heart endothelial cell lysate
Other Notes
Replaces: 05-811
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| clone | monoclonal |
| species reactivity | bovine |
| manufacturer/tradename | Upstate® |
| technique(s) | western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NM_000603 |
| UniProt accession no. | P29474 |
| shipped in | dry ice |
| target post-translational modification | phosphorylation (pThr495) |
| Gene Information | mouse ... Nos3(287024) |
