General description
Upf1 is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. Upf1 is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping.
~125 kDa
Immunogen
KLH-conjugated synthetic linear peptide corresponding to Upf1.
Application
Anti-Upf1 Antibody is a rabbit polyclonal antibody for detection of Upf1 also known as ATP-dependent helicase RENT1 ,Nonsense mRNA reducing factor 1, Up-frameshift suppressor 1 homolog & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Binding Protein (RBP)
Biochem/physiol Actions
This antibody detects Upf1.
Physical form
Affinity purified
Format: Purified
Purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
U2OS cell lysate
Evaluated by western blot on U2OS cell lysate.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected Upf1 in U2OS cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | mouse, bovine, canine, rat |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_002902.2 |
| UniProt accession no. | Q92900 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... UPF1(5976) |
