General description
PACSINs, also known as syndapins, are a protein family consisting of three members that have been implicated in synaptic vesicular membrane trafficking and regulation of dynamin-mediated endocytotic processes (Cuajungco, 2006). It is presumed that all isoforms bind Dynamin, Synaptojanin 1 and N-WASP through their Src homology 3 domains. While PACSIN1 appears to be restricted to neural tissues, two PACSINs are more widespread with PACSIN2 being ubiquitously expressed and PACSIN3 present mainly in skeletal muscle, heart and lung (Modregger, 2001). PACSIN1 has been implicated as playing a central role in synaptic vesicle recycling (Kessels, 2004). Expected molecular weight is 51 kDa.
~57 kDa
Immunogen
KLH conjugated synthetic peptide selected from the N-terminal region of human PACSIN1.
Application
Anti-PACSIN1 Antibody, N-terminus detects level of PACSIN1 & has been published & validated for use in WB, IH(P).
Biochem/physiol Actions
Reactivity with other species has not been tested.
The antibody recognizes human PACSIN1.
Physical form
Format: Purified
PBS containing 0.09% NaN3.
Preparation Note
Maintain at 2-8ºC for up to 6 months from date of receipt. -20ºC is recommended for long term storage.
Analysis Note
Evaluated on a representative lot by Western blot on mouse brain lysate using a 1:100 - 1:500 dilution of Anti-PACSIN1.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human, mouse |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| UniProt accession no. | Q9BY11 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PACSIN1(29993) |
