General description
Histone deacetylase 1 is a nuclear enzyme encoded by the Hdac1 gene (also known as HD1, RPD3, MommeD5, Hdac1-ps) in mouse and HDAC1 (also known as GoON-10, RPD3L1) gene in human. Hdac1 plays an important role in epigenetic regulation of gene expression by catalyzing the hydrolysis of N(6)-acetylated histone lysine residues. Hdac1 is also known to deacetylate p53 and thereby downregulate p53 activity.
~55 kDa observed
Immunogen
KLH-conjugated, synthetic peptide (C-EEKPEAKGVKEEVKLA) corresponding to amino acids 467-482 of mouse HDAC1. The immunizing sequence is identical in human.
Application
Anti-HDAC1 Antibody, clone 2E10 is an antibody against HDAC1 for use in Western Blotting, Immunoprecipitation.
Immunoprecipitation Analysis A representative lot was used to immunoprecipitate HDAC1 from HeLa cell lysate, followed by immune complex enzyme activity assay using HDAC Assay Kit (Fluorometric Detection), Cat. No. 17-356.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected HDAC1 in 10 µg of A431 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Replaces: 05-100
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 2E10, monoclonal |
| species reactivity | mouse, human |
| manufacturer/tradename | Upstate® |
| technique(s) | immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_032254 |
| UniProt accession no. | O09106 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... HDAC1(3065) mouse ... Hdac1(433759) |
