Immunogen
Human fascin
Application
Research Sub Category
Cytoskeleton
This Anti-Fascin Antibody, clone 55K2 is validated for use in WB, IC, IH(P) for the detection of Fascin.
Research Category
Cell Structure
Western blot. Recognizes a protein of 55 kDa. Suggested working dilution is 1:100-1:1,000. Suggested blocking buffer is 3% BSA in PBS or 3% non fat milk in PBS.
Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000.
Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Fascin. The antibody is strongly positive when antigen-presenting dendritic cells are matured. MAB3582 is a sensitive marker for Reed-Sternberg Cells of Hodgkin′s Disease. The protein is an actin bundling protein specifically localized in filopodia and growth cones.
Physical form
Format: Purified
Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt.
Analysis Note
Control
POSITIVE CONTROL:
Hela cells, neuronal tissues, antigen presenting dendritic cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 55K2, monoclonal |
| species reactivity | echinoderm, sea urchin, gerbil, human |
| species reactivity (predicted by homology) | mammals |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG1 |
| suitability | not suitable for immunoprecipitation |
| NCBI accession no. | NM_003088.2 |
| UniProt accession no. | Q16658 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... FSCN1(6624) |
