General description
iNOS/NOS II, also known as Nitric oxide synthase inducible, Hepatocyte NOS, HEP-NOS, Inducible NO synthase, Inducible NOS, iNOS, NOS type II, Peptidyl-cysteine S-nitrosylase , and encoded by the gene name NOS2 and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2). iNOS/NOS II is expressed in the liver, retina, bone cells and airway epithelial cells of the lung.
~130 kDa observed. Uncharacterized band(s) may appear in some lysates.
Immunogen
GST-tagged recombinant protein corresponding to human iNOS/NOS II.
Application
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected iNOS/NOS II in human cerebral cortex, human cerebellum, and human colon tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
This Anti-iNOS/NOS II antibody is validated for use in WB, IH for the detection of iNOS/NOS II.
Physical form
Mouse monoclonal IgG1λ in buffer containing 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in human small intestine tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected iNOS/NOS II in 10 µg of human small Intestine tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| clone | 13F5.1, monoclonal |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1λ |
| NCBI accession no. | NP_000616 |
| UniProt accession no. | P35228 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... NOS2(4843) |
