General description
Silent synapses, or excitatory synapses that lack functional -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs), are thought to be critical for regulation of neuronal circuits and synaptic plasticity. SynGAP, an excitatory synapse-specific RasGAP, regulates AMPAR trafficking, silent synapse number, and excitatory synaptic transmission in hippocampal and cortical cultured neurons. Over expression of SynGAP in neurons results in a remarkable depression of AMPAR-mediated miniature excitatory postsynaptic currents, a significant reduction in synaptic AMPAR surface expression, and a decrease in the insertion of AMPARs into the plasma membrane. Synaptic transmission is increased in neurons from SynGAP knockout mice as well as in neuronal cultures treated with SynGAP small interfering RNA.
Immunogen
peptide (KRLLDAQRGSFPPWVQQTRV) corresponding to amino acids 1289-1308 of rat SynGAP-a
Application
Anti-SynGAP Antibody detects level of SynGAP & has been published & validated for use in IC & WB.
Immunocytochemistry: 0.1 µg/mL has been reported to immunostain SynGAP in neuron cultures fixed with 4% paraformaldehyde, 4% sucrose and perme-abilized with 0.1% Triton X-100 (2).
Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Biochem/physiol Actions
SynGAP
Physical form
Format: Purified
Protein A purified
Purified in PBS with 0.05% NaN3 and 30% Glycerol
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt.
Other Notes
Replaces: 04-1071
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | rat |
| manufacturer/tradename | Upstate® |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| NCBI accession no. | NM_006772.1 |
| UniProt accession no. | Q9QUH6 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SYNGAP1(8831) |
