General description
26S proteasome non-ATPase regulatory subunit 14 is also known as 26S proteasome regulatory subunit RPN11 or 26S proteasome-associated PAD1 homolog 1. This protein is expressed from the PSMD14 gene and is the metalloprotease component of the 26S proteasome that specifically cleaves ′Lys-63′-linked polyubiquitin chains. 26S proteasome non-ATPase regulatory subunit 14 has a role in the response to double-strand breaks (DSBs) by regulating non-homologous end joining (NHEJ) via cleaving ′Lys-63′-linked polyubiquitin, retaining JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. 26S proteasome non-ATPase regulatory subunit 14 is also involved in homologous recombination repair by promoting RAD51 loading. 26S proteasome non-ATPase regulatory subunit 14 is widely expressed, but found in the highest levels in heart and skeletal muscle.
~ 35 kDa observed
Immunogen
Epitope: Region near N-terminus
KLH-conjugated linear peptide corresponding to 26S proteasome regulatory subunit RPN11.
Application
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected 26S proteasome regulatory subunit RPN11 in human cardiac myocytes and rat skelatal muscle myocytes.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Cell Cycle, DNA Replication & Repair
This Anti-26S proteasome regulatory subunit RPN11, clone 7C7.1 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of 26S proteasome regulatory subunit RPN11.
Biochem/physiol Actions
Recognizes 26S proteasome regulatory subunit RPN11
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HT-1080 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected 26S proteasome regulatory subunit RPN1 in 10 µg of HT-1080 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 7C7.1, monoclonal |
| species reactivity | human, rat |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_005796 |
| UniProt accession no. | O00487 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PSMD14(10213) |
