General description
239.3 kDa (human) and 238.3 kDa (mouse) calculated.
Sortilin-related receptor (UniProt Q92673; also known as Gp250, LDLR relative with 11 ligand-binding repeats, Low-density lipoprotein receptor relative with 11 ligand-binding repeats, LR11, SorLA, SorLA-1, Sorting protein-related receptor containing LDLR class A repeats) is encoded by the SORL1 (also known as C11orf32) gene (Gene ID 6653) in human. SorLA and Sortilin are related Vps10p-domain (Vsp10p-D) family multi-ligand-binding receptors that target LPL, growth factors and peptides, as well as soluble amyloid precursor proein (sAPP) for endocytosis. Studies show that SorLA protects sAPP against proteolytic processing in compartments localized in the cell body (soma) of hippocampal neurons, whereas sortilin facilitates sAPP degradation by lysosomes localized in neurites. Hippocampal neurons lacking sortilin display reduced sAPPα production, suggesting that sortilin promotes α-secretase activity in neuronal systems. Unlike LRP1 and LRP1b, two other known sAPP-binding receptors, SorLA and Sortilin bind sAPP in a KPI domain-independent manner and their scavenging function therefore is not limited to sAPP produced from KPI-containing APP isoforms. Human SorLA is a single transmembrane (a.a. 2138-2158) protein with a 55-amino acid cytoplasmic tail (a.a. 2159-2214) and a large luminal/extracellular (a.a. 1-2137) region that contains a Vps10p-domain (Vsp10p-D; a.a. 1-731) with a signal peptide (a.a. 1-28) and a propeptide (a.a. 29-81) sequence at the N-terminal end and five BNR (bacterial neuraminidase repeat or (F/Y)WTD/b-propeller domain) repeats (a.a. 136-573). The C-terminal half of the luminal/extracellular region contains five LDL-R class B (LB) repeats (a.a. 800-1013), an EGF-like domain (a.a. 1026-1072), eleven LDL-R class A (LA) repeats (a.a. 1076-1551), and six fibronectin type-III repeats (a.a. 1557-2118).
Immunogen
Epitope: Extracellular/luminal region outside the Vps10p domain (Vsp10p-D).
Recombinant human SorLA extracellular/luminal domain.
Application
Anti-SorLA Antibody, clone 20C11 is an antibody against SorLA for use in Immunocytochemistry, Western Blotting, ELISA.
Immunocytochemistry Analysis: 5 µg/mL from a representative lot immunostained SorLA endocytic vesicles in wild-type, but not Sorl1-/-, mouse astrocytes (Courtesy of Dr. Jakob V. Larsen, MIND-centre, University of Aarhus, Denmark).
Immunocytochemistry Analysis: A representative lot detected SorLA immunoreactivity primarily located at the cell body (soma) by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized primary murine hippocampal neurons (Gustafsen, C., et al. (2013). J. Neurosci. 33(1):64-71).
Immunocytochemistry Analysis: A representative lot immunostained endocytic vesicular structures containing endocytosed sAPP by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HEK293 cells expressing exogenously transfected human SorLA (Gustafsen, C., et al. (2013). J. Neurosci. 33(1):64-71).
Immunocytochemistry Analysis: A representative lot detected the cellular localization of exogenously expressed wild-type and the FANSHY-to-6A4 mutant human SorLA by fluorescent immuncytochemistry staining of 4% paraformaldehyde-fixed, 0.5% saponin-permeabilized SH-SY5Y transfectants (Fjorback, A.W., et al. (2012). J. Neurosci. 32(4):1467-1480).
Western Blotting Analysis: A representative lot detected endogenous SorLA in sucrose gradient-fractionated mouse brain extracts as well as the expression of exogenously transfected human SorLA in HEK293 transfectants (Gustafsen, C., et al. (2013). J. Neurosci. 33(1):64-71).
ELISA Analysis: Representative lots were employed as the detection antibody for quantiating SorLA level in human brain cortical extracts as well as the level of exogenously expressed human SorLA in CHO cells (Caglayan, S., et al. (2012). Arch. Neurol. 69(3):373-379; Schmidt, V., et al. (2012). EMBO J. 31(1):187-200).
Biochem/physiol Actions
Clone 20C11 immunostained SorLA endocytic vesicles in wild-type, but not Sorl1-/-, mouse astrocytes.
Physical form
Format: Purified
Analysis Note
Evaluated by Immunocytochemistry in human SorLA-expressing HEK293 cells.
Immunocytochemistry Analysis: 4.0 µg/mL of this antibody immunostained SorLA endocytic vesicles in HEK293 cells expressing transfected human SorLA.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 20C11, monoclonal |
| species reactivity | mouse, human |
| technique(s) | ELISA: suitable,immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_003096 |
| UniProt accession no. | Q92673 |
| target post-translational modification | unmodified |
| Gene Information | human ... SORL1(6653) |
