General description
19.30 kDa (prepro-hCAP-18), 16.02 kDa (pro-hCAP-18), 4.712 kDa (FALL-39; a.a. 132-170) and 4.493 kDa (LL-37; a.a. 134-170) calculated.
Cathelicidin antimicrobial peptide (UniProt P49913; also known as 18 kDa cationic antimicrobial protein, CAP-18, hCAP-18) is encoded by the CAMP (also known as CAP18, FALL39, LL37) gene (ORF HSD26; Gene ID 820) in human. The human cathelicidin antimicrobial protein 18 (hCAP-18) is initially translated as a precursor molecule with a signal peptide (a.a. 1-30) and a propeptide (a.a. 31-131) sequence, the removal of which yields the mature hCAP-18 (a.a. a.a. 132-173). During the inflammatory response, hCAP-18 is proteolytically cleaved into the FALL-39 (a.a 132-170), which is further processed to yield the antimicrobial protein LL-37 (CAP-37; a.a. 134-170). Neutrophils store preformed hCAP-18 within specific granules and release it into inflamed tissue as a result of granule exocytosis. Once released, hCAP-18 is activated by proteinase-3-mediated cleavage, a component of neutrophil primary granules. In addition to its microbicidal and LPS-sequestering activity, LL-37 is also involved in the regulation of innate and humoral immune responses, wound healing, and tissue repair.
Immunogen
Epitope: C-terminal.
KLH-conjugated linear peptide corresponding to the C-terminal sequence of human Cathelicidin/FALL-39/LL-37.
Application
Anti-Cathelicidin Antibody, clone 8C10.1 is an antibody against Cathelicidin/FALL-39/LL-37 for use in Immunohistochemistry.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cathelicidin/FALL-39/LL-37 immunoreactivity in human spleen tissue sections.
Research Category
Signaling
Research Sub Category
Inflammation & Autoimmune Mechanisms
Biochem/physiol Actions
Clone 8C10.1 targets a C-terminal epitope present in the prepro- (a.a. 1-173) and pro- (a.a. 31-173) forms of hCAP-18, as well as the processed antibacterial peptides FALL-39 (a.a 132-170) and LL-37 (a.a. 134-170), but not the preprodomain (a.a. 1-131) or prodomain (a.a. 31-131) fragment.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunohistochemistry in human bone marrow tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Cathelicidin/FALL-39/LL-37 immunoreactivity in human bone marrow tissue sections.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 8C10.1, monoclonal |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_004336 |
| UniProt accession no. | P49913 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CAMP(820) |
