General description
150 kDa calculated.
Etanercept is a chimeric fusion protein consisting of the extracellular ligand-binding portion of the p75 TNF receptor linked to the Fc portion of human IgG1. This Fc component contains the hinge, CH2 and CH3 regions, but not the CH1 region of IgG1. Etanercept binds to TNF-alpha and blocks its interactions with receptors. Hence, it is considered a TNF inhibitor that functions as a decoy receptor that binds to TNF. Although it mimics the inhibitory effects of naturally occurring soluble TNF receptors, the difference is that Etanercept has a greatly extended half-life in the bloodstream. Its speci fic activity is reported to be about 1.7 x 106 units/mg. Etanercept is shown to modulate biological responses produced by TNF, including expression of adhesion molecules responsible for leukocyte migration, serum levels of cytokines, and serum levels of matrix metalloproteinase-3. It is also shown to affect animal models of inflammation, including murine collagen-induced arthritis. Clone ETA63C8 can bind to Etanercept and block its activity.
Immunogen
Chimeric fusion protein cotaining the extracellular ligand-binding portion of the p75 TNF receptor linked to the Fc portion of human IgG1.
Application
Anti-Etanercept, clone ETA63C8, Cat. No. MABF1973, is a mouse monoclonal antibody that can bind to Etanercept and has been tested for use in ELISA and Neutralizing.
Neutralizing Analysis: A representative lot neutralized Etanercept in Neutralizing applications (Detrez, I., et. al. (2016). J Immunol. 196(6):2879-84).
ELISA Analysis: A representative lot detected Etanercept in ELISA applications (Detrez, I., et. al. (2016). J Immunol. 196(6):2879-84).
ELISA Analysis: 4 µg/mL from a representative lot detected Etanercept in human serum with AS (ankylosing spondylitis) (Courtesy of Professor Ann Gils, Ph.D., KU Leuven Belgium).
Research Category
Inflammation & Immunology
Biochem/physiol Actions
Clone ETA63C8 detects Etanercept in human samples. It can bind to Etanercept and block its activity.
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | ETA63C8, monoclonal |
| species reactivity | human |
| packaging | antibody small pack of 25 μL |
| technique(s) | ELISA: suitable,neutralization: suitable |
| isotype | IgG1κ |
| target post-translational modification | unmodified |
