General description
Delta-type Opioid Receptor (D-OR-1), also known as DOR-1, and encoded by the gene name OPRD1/ OPRD, is a G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Binding of Delta-type Opioid Receptor with ligands causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Delta-type Opioid Receptor signaling has been shown to lead to the inhibition of adenylate cyclase activity. Recent studies have also demonstrated that Delta-type Opioid Receptor inhibits the neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Delta-type Opioid Receptor also may play a role in the perception of pain and in opiate-mediated analgesia. Additionally, it may play a role in developing analgesic tolerance to morphine, and it may form homooligomers. Delta-type Opioid Receptor interacts with GPRASP1. Delta-type Opioid Receptor is detected in oocytes (at protein level), and in brain cortex, hypothalamus, hippocampus and olfactory bulb.
~65 kDa observed. The calculated molecular weight of this protein is 40 kDa, but can be observed at ~65 kDa due to high glycosylation.
Immunogen
Epitope: C-terminus
GST-tagged recombinant protein corresponding to the C-terminus of human delta-type Opioid Receptor.
Application
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected delta-type Opioid Receptor in human cerebral cortex, human uterus, and human thalamus tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
This Anti-delta-type Opioid Receptor antibody is validated for use in WB, IH(P) for the detection of delta-type Opioid Receptor.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected delta-type Opioid Receptor in 10 µg of mouse brain tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 4E6.2, monoclonal |
| species reactivity | human, mouse |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_000902 |
| UniProt accession no. | P41143 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... OPRD1(4985) |
