General description
Src-like-adapter/SLAP1 protein, also known as SLAP, and encoded by the gene SLA/SLAP/SLAP1, is an adapter protein which negatively regulates T cell receptor signaling by inhibiting T cell antigen induced proliferation and works to control the positive selection and mitosis of T cells. SLAP1 also acts to connect signaling proteins such as ZAP70 with the T cell specific CBL ubiquitin ligase that plays a central role in degrading signaling proteins, and a critical role in limiting or initiating the T-cell specific- antigen-specific immunotolerance limits in T cells activation. Src-like-adapter/SLAP1 is localized to the cytoplasm and endosomes within cells. Src-like-adapter/SLAP1 is expressed in lung and fetal brain, and weakly expressed in heart, adult brain, placenta, liver, skeletal muscle, kidney and pancreas. Recent research suggest that SLAP1 may also play a role in different cancers and in modulation of receptor tyrosine kinase signaling in cancers apart from its conventional role in regulation of T cell receptor stability.
~38 kDa observed. The calculated molecular weight of this protein is 32 kDa; however, it may be observed at higher molecular weights due to known Post-translational modification sites.
Immunogen
Linear peptide corresponding to human Src-like-adapter/SLAP1.
Application
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
This Anti-Src-like-adapter Antibody/SLAP1, clone 1D9.1 is validated for use in western blotting for the detection of Src-like-adapter/SLAP1.
Physical form
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in Human Lung tissue lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Src-like-adapter/SLAP1 in 400 µg of human lung tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 1D9.1, monoclonal |
| species reactivity | human |
| technique(s) | western blot: suitable |
| isotype | IgMκ |
| NCBI accession no. | NP_006739 |
| UniProt accession no. | Q13239 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SLA(6503) |
