General description
Rho-related GTP-binding protein RhoB (UniProt: P62745; also known as Rho cDNA clone 6, h6) is encoded by the RHOB (also known as ARH6, ARHB) gene (Gene ID: 388) in human. RhoB is a member of the Ras super-family of small GTP-binding proteins, which is involved in hydrolysis of GTP and is active in GTP-bound form. RhoB is a short-lived Rho GTPase whose expression is inducible by a variety of stimuli including growth factors and stress stimuli. Its levels are up-regulated by DNA damaging agents, such as hydrogen peroxide or ionizing radiation. RhoB plays a negative role in tumorigenesis as its deletion is known to cause tumor formation. It is reported to mediate apoptosis in neoplastically transformed cells following DNA damage. It is not considered to be essential for development, but affects cell adhesion and growth factor signaling in transformed cells. RhoB displays 83% homology with RhoA. However, unlike RhoA, which is cytosolic and translocates to plasma membrane upon activation, RhoB localizes to endosomes/multivesicular bodies. RhoB participates in sorting and degradation of growth factors and cytokine receptors. RhoB has three nucleotide-binding regions (aa 12-19; 59-63; and 117-120). RhoB is shown to be critically required for the inflammatory response of endothelial cells to TNF alpha, possibly through MAP kinase activation downstream of the TNFR.
~20 kDa observed; 22.12 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human RhoB.
Application
Anti-RhoB, clone 7F7, Cat. No. MABS1990, is a mouse monoclonal antibody that detects Rho-related GTP-binding protein RhoB in human and has been tested for use in ELISA and Western Blotting.
Research Category
Signaling
Western Blotting Analysis: A 1:2,000 dilution from a representative lotdetected RhoB in 10 µg of MCF7 cell lysate.
Western Blotting Analysis: A representative lot detected RhoB in mouse and rabbit spleen, mouse kidney, recombinant RhoB, 293 cells expressing RhoB, CHO, H9C2, PK1P.185, COS, MDA MB-231, MCF7 and chicken fibroblasts (Courtesy of Lisa Laury-Kleintop, Ph.D., Lankenau Institute for Medical Research, Philadelphia PA, USA).
ELISA Analysis: A representative lot detected RhoB in Plate bound recombinant RhoB (Courtesy of Lisa Laury-Kleintop, Ph.D., Lankenau Institute for Medical Research, Philadelphia PA, USA).
Biochem/physiol Actions
Clone 7F7 detects RhoB in multiple species. It targets an epitope within 19 amino acids from the C-terminal half. This clone specifically detects RhoB, but not RhoA, Rac1, or cdc42.
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected RhoB in 10 µg of HEK293 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 7F7, monoclonal |
| species reactivity | rat, mouse, hamster, porcine, human, chicken, monkey, rabbit |
| packaging | antibody small pack of 25 μL |
| technique(s) | ELISA: suitable,western blot: suitable |
| isotype | IgG1κ |
| web metadata keyword.default | Anti-RhoB Antibody clone 7F7 Sigma-Aldrich,Anti-RhoB antibody for protein interaction studies,Anti-RhoB antibody,ELISA grade Anti-RhoB antibody 7F7,RHOB antibody clone 7F7 for research,RHOB gene antibody clone 7F7 Sigma-Aldrich,Rho-related GTP-binding protein antibody,RhoB GTPase antibody for western blot,RhoB monoclonal antibody for immunofluorescence,anti-RhoB antibody for cell signaling studies,high purity Anti-RhoB antibody for ELISA,monoclonal Anti-RhoB antibody 7F7,recombinant RhoB antibody for western blot |
| NCBI accession no. | NP_004031.1 |
| UniProt accession no. | P62745 |
| target post-translational modification | unmodified |
| Gene Information | human ... RHOB(388) |
