General description
Ribosyldihydronicotinamide dehydrogenase [quinone] (EC 1.10.5.1; UniProt P16083; also known as NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, Quinone reductase 2, QR2) is encoded by the NQO2 (also known as NMOR2) gene (Gene ID 4835) in human. NQO1 (EC 1.6.5.2) and NQO2 (EC 1.10.5.1; formerly EC 1.10.99.2) constitute the quinone oxidoreductase family in human. NQO1 is involved in vitamin K metabolism and in reducing the cellular quinone concentration to prevent the build-up of cellular reactive oxygen species (ROS). Both enzymes contain a tightly bound FAD as the oxidant cofactor, but differ in their reductant cofactors utilization. While NQO1 can efficiently utilize NADH or NADPH, NQO2 exhibits only low activity in the presence of NADH or NADPH. Instead, non-physiological compounds, such as dihydronicotinamide riboside (NRH), N-methyldihydronicotinamide, and dihydrobenzylnicotamide, are reported to act as good reductant cofactors in NQD2-catalyzed reactions in vitro. NQO1 & NQO2 catalyzed the reduction of a wide variety of substrates, including quinones, nitro-compounds, and iron (III) ions. NQO1 is inhibited by the anticoagulant dicoumarol, while NQO2 is only weakly inhibited by dicoumarol and more strongly inhibited by resveratrol.
~26 kDa observed. Uncharacterized band(s) may appear in some lysates.
Immunogen
Epitope: Internal (N-terminal half).
GST-tagged recombinant human NQO2 N-terminal fragment.
Application
Anti-NQO2 Antibody, clone 4A4.1 is an antibody against NQO2 for use in Western Blotting, Immunohistochemistry.
Research Category
Apoptosis & Cancer
Research Sub Category
Oxidative Stress
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected NQO2 in 10 µg of Jurkat cell lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NQO2 in human kidney, liver, and cerebral cortex tissue sections.
Biochem/physiol Actions
Clone 4A4.1 recognizes an internal epitope within the N-terminal half of human Quinone reductase 2.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected NQO2 in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 4A4.1, monoclonal |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_000895 |
| UniProt accession no. | P16083 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... NQO2(4835) |
