Immunogen
Partially purified platelet glycoprotein IIb/lIIa complex.
Application
Anti-Integrin β3 Antibody, clone BB10 is an antibody against Integrin β3 for use in WB, IC, IH.
Immunoblotting: 1:1000 (Ylanne et al., 1988, 1990). The antibody is most effective when used against non-reduced samples.
immunohistochemistry (indirect immunofluorescence)1:200 - 1:400. (Ylanne et al., 1988).
Does not work on paraffin embedded sections.
Final working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts with the beta-3 subunit of the integrin family, including GPIIIa of platelets. Recognized an extracellular epitope of beta-3.
Physical form
Format: Purified
Purified immunoglobulin. In PBS with 1% BSA and 0.1% sodium azide.
Preparation Note
Maintain between 2 and 8°C up to 12 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | BB10, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1 |
| suitability | not suitable for immunohistochemistry (Paraffin) |
| NCBI accession no. | NM_000212.2 |
| UniProt accession no. | P05106 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ITGB3(3690) |
