General description
Millipore is pleased to offer our new 96-well ELISA Rat/Mouse NPY kit. This kit requires no sample extraction and supports our commitment to the study of various metabolic disease markers.
Neuropeptide Y (NPY) is a 36-amino acid peptide secreted by the hypothalmus. It has been associated with a number of physiologic processes, including the regulation of energy, balance, memory and learning. In addition to increasing appetite, the presence of NPY increases the proportion of energy stored as fat and blocks signals to the brain. NPY is closely related to other peptide hormones such as pancreatic polypeptide, PYY and correlates nicely to Leptin levels.
Application
Research Category
Metabolism
Research Sub Category
Obesity
Metabolic Disorders
This assay requires 20 µl of diluted plasma and is performed at room temperature as a 3 hour assay.
Used to detect/quantify: NPY
Biochem/physiol Actions
Cross Reactivty
Refer to kit protocol for details
Other Notes
Please contact Technical Service for linearity of dilution.
Disclaimer
For research use only. Not for use in diagnostic procedures.
| Quality Level | 200 |
| species reactivity | rat, mouse |
| packaging | kit of 1 × 96 wells |
| parameter | 20 μL sample volume (4hr assay) |
| technique(s) | ELISA: suitable |
| input | sample type plasma (K2 EDTA) |
| UniProt accession no. | P01303 |
| application(s) | research use |
| assay range | accuracy: 89-93%,sensitivity: 0.002 ng/mL (20 μl sample size),standard curve range: 0.01-2 ng/mL,inter-assay cv: 6-12% intra-assay cv: 1-3% |
| detection method | colorimetric (450nm/590nm) |
| shipped in | wet ice |
| storage temp. | 2-8°C |
