General description
Rod cGMP-specific 3′,5′-cyclic phosphodiesterase subunit alpha (UniProt: P16499; also known as EC: 3.1.4.35, cGMP-PDE alpha, PDE V-B1) is encoded by the PDE6A (also known as PDEA) gene (Gene ID: 5145) in human. cGMP-PDE alpha is expressed in cells of the retinal rod outer segment. cGMP-PDE participates in processes of transmission and amplification of the visual signal. This holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by cGMP-PDE alpha, cGMP hydrolysis, and guanylate cyclase cGMP synthesis. cGMP-PDE alpha has five metal ion binding sites that bind two divalent metal cations per subunit. Site 1 preferentially binds zinc ions, while site 2 has a preference for magnesium and/or manganese ions. cGMP-PDE alpha has two GAF domains (aa 73-222 GAF1 and aa 254-431 GAF2). Mutations in PDE6A gene have been linked to retinitis pigmentosa that is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors.
~100 kDa observed; 99.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
GST/His-tagged recombinant fragment corresponding to 77 amino acids from the N-terminal region of human Rod cGMP-specific 3′,5′-cyclic phosphodiesterase subunit alpha (cGMP-PDE alpha).
Application
Anti-PDE6a, clone 7A11.1, Cat. No. MABN2250, is a highly specific mouse monoclonal antibody that targets cGMP-PDE alpha and has been tested for use in Western Blotting.
Biochem/physiol Actions
Clone 7A11.1 is a mouse monoclonal antibody that specifically detects Rod cGMP-specific 3′,5′-cyclic phosphodiesterase subunit alpha (cGMP-PDE alpha) in human retinal tissue. It targets an epitope within 77 amino acids from the N-terminal region.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in human retinal tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PDE6a in Human retinal tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | mouse |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 7A11.1, monoclonal |
| species reactivity | human |
| packaging | antibody small pack of 25 μg |
| technique(s) | western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_000431 |
| UniProt accession no. | P16499 |
| target post-translational modification | unmodified |
| Gene Information | human ... PDE6A(5145) |
