产品详情

pCDFDuet-1 DNA - Novagen，10UG，71340-3CN，Sigma

销售价： ¥ 4978.05 / 件
优    惠： 请询价
订货号 ： 0BV8771
品牌型号 ： 71340-3CN
货期 ： 询货期
最小订货量 ： 1件
产品介绍 Product Description

General description

pCDFDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet-1, pACYCDuet-1, and pRSFDuet-1 or pCOLADuet-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.


Application

pCDFDuet-1 DNA has been used as a vector for the expression of recombinant proteins in E-coli cells.


Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany


Disclaimer

Toxicity: Standard Handling (A)


技术参数 Specifications
formliquid
Quality Level100
manufacturer/tradenameNovagen®
storage conditionOK to freeze
promoterPromoter name: T7
selectionspectinomycin
storage temp.−70°C
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