Immunogen
His tagged recombinant protein.
Application
Detect Polyhistidine Tag using this Anti-Polyhistidine Tag Antibody validated for use in ELISA & WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Western blot: 1:2,000
ELISA
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts with the epitope MRGSHHHHHH which is present in pQE vectors. It is expected that the antibody will also react with proteins expressed from vectors such as PRSET A, B, and C. The antibody should not react with pAC, N, T, A, B, C baculovirus vectors.
Physical form
Format: Purified
Purified immunoglobulin. Liquid containing 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB3112
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | monoclonal |
| species reactivity (predicted by homology) | all |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,western blot: suitable |
| isotype | IgG1 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
