General description
The p32 protein is a doughnut-shaped trimer that is primarily localized in the mitochondria. It has also been reported to be present at the cell surface and in the nucleus. The role of p32 in mammalian cells is unclear. The yeast p32 homologue has been reported to regulate oxidative phosphorylation, and a link to autophagy has been proposed. Elevated expression of p32 in cancer has also been noted. Additionally, p32 is primarily localized in hypoxic/nutrient-deprived regions within tumors and that, in addition to tumor cells, a tumor-associated macrophage/myeloid cell subpopulation closely linked to tumor lymphatics expresses high levels of p32. The expression of p32 at the cell surface, where it can be targeted with p32-binding antibodies and peptides, adds a second level of tumor specificity to p32 expression. The unique expression pattern of p32 in tumor cells, tumor lymphatics, and tumor-associated macrophages/myeloid cells makes p32 a potential target for the diagnosis and therapy of cancer.
~ 32 kDa
Immunogen
Cocktail of peptides corresponding to the N-terminus of human and mouse p32.
Epitope: Unknown
Application
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected p32 in HeLa and A431 cells.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-p32 Antibody is validated for use in WB, IC for the detection of p32.
Biochem/physiol Actions
This antibody recognizes p32.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
A431 cell lysate
Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected p32 on 10 µg of A431 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, rat, human |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NP_001203 |
| UniProt accession no. | Q07021 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... C1QBP(708) |
