General description
Protein fat-free homolog (Fat Free), also known as Vacuolar protein sorting-associated protein 51 homolog and Another new gene 2 protein (ANG2). Fat Free is a component of the Golgi-associated retrograde protein (GARP) complex which mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). The GARP complex maintains protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. Fat Free participates in acid hydrolase receptor transport, likely by promoting tethering and fusion of endosome-derived carriers to the TGN.
~80 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~73 kDa and ~86 kDa
Immunogen
KLH-conjugated linear peptide corresponding to human Fat Free.
Application
Anti-Fat Free Antibody is a highly specific rabbit polyclonal antibody, that targets Fat free & has been tested in western blotting.
Research Category
Signaling
Research Sub Category
Lipid Metabolism & Weight Regulation
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse brain tissue lysate
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Fat Free in 15 µg of mouse brain tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse |
| species reactivity (predicted by homology) | human (immunogen homology) |
| technique(s) | western blot: suitable |
| UniProt accession no. | Q9UID3 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ANG(283) |
