General description
The inhibitory neurotransmitter, GABA, acts through two ligand-gated channels, GABA(A) and GABA© receptors and GABA(B), the latter of which acts through a G protein mechanism. GABA(B)R2 is one of the two G-protein coupled receptors for GABA(B) that acts as a heteromeric assembly with GABA(B)R1.
Immunogen
Epitope: a.a. 42-54 rat
peptide residues 42-54 of the rat 105 kDa GABA(b)R2 protein
Application
Immunoblotting: 1:500-1:1,000. Membrane preparations with proteinase inhibitors are recommended. Whole rat brain membrane preparations. The antibody detects a ~105kDa band under standard western conditions. The immunogen used is 100% conserved in human by sequence analysis; other species have not been examined for reactivity or homology.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-GABA B Receptor R2 Antibody, a.a. 42-54 rat is validated for use in WB for the detection of GABA B Receptor R2.
Biochem/physiol Actions
GABAB Receptor R2 subunit, N-terminus. No reactivity with GABABR1 has been detected.
Physical form
Format: Purified
Purified immunoglobulin. Liquid. Buffer = PBS containing 0.02% sodium azide.
Preparation Note
Maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human, rat |
| manufacturer/tradename | Chemicon® |
| technique(s) | western blot: suitable |
| NCBI accession no. | NM_005458.5 |
| UniProt accession no. | O75899 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... GABBR2(9568) |
