General description
Note: 1 KU = 1000 units.
Recombinant, rabbit glycogen synthase kinase 3β expressed in E. coli. Glycogen synthase kinase 3β is a monomeric protein whose activity is independent of second messengers such as cAMP or IP3. Recent studies demonstrate that, like the dual specificity MAP kinase, it autophosphorylates on Ser, Thr, and Tyr. It phosphorylates several exogenous substrates, including protein phosphatase 1, the regulatory domain of protein kinase A, myelin basic protein, protein phosphatase inhibitor 2, c-jun, c-myb, c-myc, CREB, CREM, p90rsk, and microtubule-associated tau protein only on Ser and/or Thr, but not on Tyr residues. Plays a key role in Wnt and insulin signaling.
Physical form
In 50 mM NaCl, 30 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, 0.03% BRIJ®-35 Detergent, pH 7.5
Preparation Note
Following initial thaw aliquot and freeze (-20°C).
Other Notes
Dajani, R., et al. 2001. Cell105, 721.
Wang, Q.M., et al. 1995. Biochem. Biophys. Res. Commun.208, 796.
Fiol, C.J., et al. 1994. J. Biol. Chem.269, 32187.
Wang, Q.M., et al. 1994. J. Biol. Chem.269, 14566.
Wang, Q.M., et al. 1994. Biochemistry33, 143.
deGroot, R.P., et al. 1993. Oncogene8, 841.
Ishiguro, K., et al. 1993. FEBS Lett.325, 167.
One unit is defined as the amount of GSK-3 required to catalyze the transfer of 1 pmol phosphate to CREB phosphopeptide KRREILSRRPS(P)YRP (200 µM), in 1 min at 30°C in a reaction volume of 25 µl.
Legal Information
Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| recombinant | expressed in E. coli |
| Quality Segment | 100 |
| form | liquid |
| specific activity | ≥250,000 units/mL |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| foreign activity | DNase <10% (conversion of RFI DNA to RFII and linear forms),Phosphatases and Proteases, none detected,RNases <10% (RNA degradation) |
| storage temp. | −20°C |
