General description
Rab5A is a membrane associated Ras related GTPase that is required for endosome fusion with the plasma membrane. Rab5A also contributes to filopodia extension and other cellular cytoplasmic extensions. Rab5A plays important roles in cellular differentiation, neurogenesis, exocytosis and protein transport. Rab proteins are a large family of GTP-binding proteins that regulate cellular membrane traffic and organelle identity. When membrane bound, each Rab has a very specific cellular location and recognizes distinct subsets of intracellular membranes, which allows Rab proteins to act as as key cellular regulators, determining the recruitment of downstream effectors to the correct membrane at the correct time. Recent research indicates that the localization of Rab proteins happens by sensing the stored curvature elastic energy in the membrane and the specific RabGEFs (Rab-activating guanosine diphosphate/guanosine triphosphate exchange factors) that associate with a particular Rab. Rab5A associates with Rabex-5 and this association provides the determinants for Rab5A specific membrane targets.
~24 kDa observed.
Immunogen
KLH-conjugated linear peptide corresponding to human Rab5A.
Application
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Rab5A in human testis tissue.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
This Anti-Rab5A Antibody, clone 7D8.1 is validated for use in Western Blotting and Immunohistochemistry for the detection of Rab5A.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HEK-293 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Rab5A in 10 µg of HEK-293 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 300 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 7D8.1, monoclonal |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_004153 |
| UniProt accession no. | P20339 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... RAB5A(5868) |
