General description
Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.
Product Source: N-terminal GST-tagged, recombinant, human JAK1 amino acids 866-end, expressed by baculovirus in Sf21 insect cells. Activated in vitro by auto-phosphorylation.
Application
Research Category
Signaling
Biochem/physiol Actions
Target Sub-Family: TK
Packaging
Also available in 250μg size as catalog # 14-918M and in bulk as catalog # 14-918-K.
Physical form
50mM Tris/HCl pH7.5, 150mM NaCl, 5mM β-glycerophosphate, 0.5mM sodium orthovanadate, 50% v/v glycerol, 0.02% Triton™ X-100, 0.5mM EDTA, 2mM DTT. Frozen solution.
Purified using glutathione sepharose.
Preparation Note
6 months at -70°C
Analysis Note
Routinely evaluated by phosphorylation of peptide GEEPLYWSFPAKKK.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | human |
| Quality Level | 100 |
| recombinant | expressed in baculovirus infected Sf21 cells |
| mol wt | Mw 61 kDa |
| manufacturer/tradename | Upstate® |
| technique(s) | activity assay: suitable (kinase) |
| NCBI accession no. | NM_002227 |
| UniProt accession no. | P23458 |
| Gene Information | human ... JAK1(3716) |
