General description
125 and 150 kDa calculated
Collagens are major fibrous structural elements of cartilage, tendon, bone, skin, lung, and blood vessels. Type I collagen, a heterotrimer consisting of two α1 chains and one α2 chain, is the most abundant fibrillar collagen. It is preferentially synthesized in bone, skin, and tendon and is the most abundant protein found in these tissues.
Immunogen
Epitope: C-terminus
Linear peptide corresponding to the C-terminus of human Collagen type I alpha 2.
Application
Activity Assay Analysis: A representative lot from an independent laboratory inhibits fibrin formation in vitro (Chung, H. J., et al. (2008). J Biol Chem. 283(38):25879-25886.).
Anti-COL1A2 antibody, clone alpha2CT, Azide Free is an antibody against COL1A2 for use in IHC & activity assay.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Physical form
Format: Purified
Protein L
Purified mouse monoclonal IgAκ in buffer containing PBS without preservatives.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Immunohistochemistry Analysis in human bone tissues.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected COL1A2 in human bone tissues.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | alpha2CT, monoclonal |
| species reactivity | human |
| technique(s) | activity assay: suitable,immunohistochemistry: suitable |
| isotype | IgAκ |
| NCBI accession no. | NP_000080 |
| UniProt accession no. | P08123 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ANXA6(309) |
