General description
Full length, recombinant, human histone deacetylase 6 (HDAC6) fused to GST at the N-terminus and expressed in S. frugiperda insect cells using a baculovirus expression system. Actively maintained in the cytoplasm, HDAC6 is uniquely dependent on its ubiquitin-binding domain and functions at the nexus between two cellular signaling systems, involving protein acetylation and ubiquitination. The active deacetylase is useful in the study of HDAC6 regulation and for inhibitor screening.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
40 mM Tris-HCl pH8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween 20, 3mM DTT, and 20% glycerol.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
One unit is defined as the amount of enyme that is required to deacetylate 1 pmol fluorescent HDAC peptide substrate per min at 30°C.
Pandey, U.B., et al. 2007. Nature447, 859.
Hubbert, C., et al. 2002. Nature417, 455.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| recombinant | expressed in insect cells (S. frugiperda) |
| Quality Segment | 100 |
| assay | ≥80% (SDS-PAGE) |
| form | liquid |
| specific activity | ≥50 units/μg protein |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| shipped in | wet ice |
| storage temp. | −70°C |
