General description
68.66 kDa calculated.
Sodium/iodide cotransporter (UniProt: Q92911; also known as Na(+)/I(-) cotransporter, Sodium-iodide symporter, Na(+)/I(-) symporter) is encoded by the SLC5A5 (also known as NIS) gene (Gene ID: 6528) in human. Sodium/iodide cotransporter is a multi-pass membrane protein that mediates iodide uptake in thyroid gland. It is expressed primarily in thyroid tissue, but is present in lower levels in mammary gland and ovary. It expression is significantly reduced in tumors. The presence of symporter in mammary glands leads to secretion of iodine in milk that is required for thyroid function in neonatal animals. NIS gene and protein expression is stimulated by thyroid-stimulating hormone. Both human and rat NIS contain N-glycosylation sites, however, glycosylation is not required for its full activity. Abnormalities in the expression of NIS gene lead to thyroid disease and can result in congenital hypothyroidism. Autoantibodies to NIS adversely affect iodide transport.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human Sodium-iodide symporter.
Application
Anti-Sodium-Iodide Symporter, C-terminal 2, Cat. No. ABC1454, is a rabbit polyclonal antibody that detects Sodium/iodide cotransporter and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Sodium-Iodide Symporter in human thyroid and human salivary gland tissue sections.
Immunocytochemistry Analysis: A representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with wild-type human NIS and in COS-7 cells transfected with mutant Q267E human NIS (De la Vieja, A., et. al. (2005). Mol Endocrinol. 19(11):2847-58; De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).
Immunohistochemistry Analysis: A representative lot detected Sodium-Iodide Symporter in Immunohistochemistry application (De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87; Wapnir, I.L., et. al. (2004). Clin Cancer Res. 10(13):4294-302; Wapnir, I.L., et. al. (2003). J Clin Endocrinol Metab. 88(4):1880-8).
Immunoprecipitation Analysis: A representative lot detected Sodium-Iodide Symporter in Biotinylated NIS constructs expressed in COS-7 cells (De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).
Flow Cytometry Analysis: A representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with WT and mutant Q267E NIS (De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with hNIS (Courtesy of Dr Nancy Carrasco & Dr. Andrea Reyna-Neyra at Yale University).
Western Blotting Analysis: A representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with NIS and COS-7 cells transfected with WT and mutant Q267E NIS (De la Vieja, A., et. al. (2005). Mol Endocrinol. 19(11):2847-58; De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).
Immunohistochemistry Analysis: A representative lot detected Sodium-Iodide Symporter in IHC-P human salivary gland and breast tissues (Tazebay, U.H., et. al. (2000). Nat Med. 6(8):871-8).
Research Category
Apoptosis & Cancer
Biochem/physiol Actions
This rabbit polyclonal antibody detects Sodium-iodide symporter in human. It targets an epitope within 13 amino acids from the C-terminal end. For Western blotting application, please do not boil the sample to avoid any aggregation. When transfering protein to nitrocellulose membrane use of 0.05% (w/v) lithium dodecyl sufate is highly recommended in the transfer buffer.
Physical form
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 76 mM Tris, 192 mM glycine, pH 7.4 with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunohistochemistry in human stomach tissue sections.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Sodium-Iodide Symporter in human stomach tissue sections.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human |
| technique(s) | flow cytometry: suitable,immunohistochemistry: suitable (paraffin),immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NP_000444 |
| UniProt accession no. | Q92911 |
| target post-translational modification | unmodified |
| Gene Information | human ... SLC5A5(6528) |
