General description
Rabbit polyclonal antibody purified by ammonium sulfate precipitation. Recognizes recombinant and monocyte-derived TNF-α.
Recognizes recombinant and monocyte-derived TNF-α. Exhibits some cross-reactivity with lymphotoxin (TNF-β) and mouse TNF-α in L929 cells.
This Anti-TNF-α Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunoblotting, Neutralization Studies, Paraffin Sections, Radioimmunoassay for the detection of TNF-α.
Immunogen
recombinant, human TNF-α
Application
ELISA (see comments)
Frozen Sections (see comments)
Immunoblotting (see comments)
Neutralization Studies (see comments)
Paraffin Sections (see comments)
Radioimmunoassay (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Analysis Note
Positive Control
L929 cells
Other Notes
1 EU corresponds to 0.1 ng endotoxin (EC-6/USP Standard)
Reacts specifically with natural and recombinant human TNF-α. An approximately 20 µg/ml solution of this antibody has been shown to neutralize 100% of the activity of 0.5 ng/ml recombinant human TNF-α, as measured in the standard L929 cell bioassay. This antibody has also been reported to work for ELISA, frozen sections, immunoblotting, paraffin sections, radioimmunoassay. Endotoxin: ≤5 EU/mg protein as determined by the Biowhittaker Endotect LAL assay. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | liquid |
| does not contain | preservative |
| species reactivity | human, mouse (weakly) |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (ELISA Frozen Sections Immunoblotting Neutralization Studies Paraffin Sections Radioimmunoassay ) |
| impurities | ≤1 EU/mg Endotoxins |
| isotype | IgG |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... TNF(7124) |
