Application
Anti-Integrin α5β1 Antibody, clone HA5 detects level of Integrin α5β1 & has been published & validated for use in FC & IP.
Immunoprecipitation of VLA-5 integrins from lysate of 10E6 cell equivalent requires 2 μg of MAB1999.
Flow cytometry: 10 μg/mL
Does not detect VLA-5 by Western blot.
MAB1999 does not affect VLA-5 mediated cell adhesion to fibronectin.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts with the fibronectin receptor VLA-5 (alpha-5beta-1) integrin complex.
Physical form
Format: Purified
Purified immunoglobulin from Protein A chromatography. Liquid in 0.02 M PB pH 7.6, 0.25 M NaCl, 0.1% sodium azide.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | mouse |
| Quality Level | 100 |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | HA5, monoclonal |
| species reactivity | pig, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | flow cytometry: suitable,immunoprecipitation (IP): suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NM_002205.2 |
| UniProt accession no. | P08648 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ITGA5(3678) |
