General description
81kDa
Ezrin is also referred to as Villin-2 and Cytovillin
Immunogen
peptide corresponding to amino acids 479-498 of human Ezrin (C-EPVSYHVQESLQDEGAEPTG)
Application
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Ezrin Antibody is validated for use in WB, IH for the detection of Ezrin.
Biochem/physiol Actions
Ezrin
Physical form
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Format: Purified
Protein A chromatography
Preparation Note
2 years at -20°C
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
routinely evaluated by immunoblot on RIPA lysates from A431 cells or a mouse brain lysate
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | mouse, human |
| manufacturer/tradename | Upstate® |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NM_003379 |
| UniProt accession no. | P15311 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Ezr(22350) |
