General description
Interleukin-1 receptor-associated kinase-like 2 (IRAK-2) binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. IRAK-2 also mediates activation of NF-κB, which is a pivotal transcription factor mediating inflammatory and immune response. Additionally, IRAK-2 may play an important role in IL-1R and TLR mediated inflammatory response. IRAK-2 is expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
~65 kDa observed. Uncharacterized bands may appear in some lysate(s).
Immunogen
KLH-conjugated linear peptide corresponding to human IRAK-2.
Application
Anti-IRAK-2 Antibody is a highly specific rabbit polyclonal antibody, that targets IRAK2 & has been tested in western blotting, ICC & Immunofluorescence.
Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected IRAK-2 in HeLa cells.
Immunofluorescence Analysis: 10 µg/mL from a representative lot detected IRAK-2 in HeLa cells.
Research Category
Inflammation & Immunology
Research Sub Category
Immunological Signaling
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa and K562 cell lysates
Evaluated by Western Blot in HeLa and K562 cell lysates.
Western Blot Analysis: 2 µg/mL of this antibody detected IRAK-2 in 15 µg of HeLa and K562 cell lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable,immunofluorescence: suitable,western blot: suitable |
| UniProt accession no. | O43187 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... IRAK2(3656) |
