Immunogen
Synthetic peptide from the intracellular C-terminal region of human dopamine transporter (CEKDRELVDRGEVRQFTLRHWL).
Application
Anti-Dopamine Transporter Antibody, C-terminus detects level of Dopamine Transporter & has been published & validated for use in IH & WB.
Immunoblotting: 0.1-1.0 μg/mL. The antibody recognizes the ~88 kDa DAT protein in Western blot of human strial samples.
Immunohistochemistry using formaldehyde-fixed brain sections.
Not recommended for use on rodent.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Dopamine Transporter (DAT), C-terminal.
Physical form
Affinity purified immunoglobulin. Liquid in 100 μL of 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.
Preparation Note
Maintain at -20 to -80°C in undiluted aliquots for up to six months after date of receipt. Avoid repeated freeze-thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | monkey, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| NCBI accession no. | NM_001044.3 |
| UniProt accession no. | Q01959 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... SLC6A3(6531) |
