General description
PSD95 (postsynaptic density95) represent a class of anchoring proteins which serve to localize various neuronal ion channels to post-synaptic densities. PSD95 and related proteins contain PDZ domains, which interact with NMDA receptors and voltage-gated K+ channels via a ET/SXV motif in the cytoplasmic carboxyl terminus of the receptor channels.
~80 kDa observed. The calculated molecular weight is 80 kDa, however phospho PSD95 (Thr19) has been shown as a ~80-95 kDa band in some western blots (Nelson, C.D., et al. (2013). J. of Neuroscience. 33(29):12122-12135).
Immunogen
Epitope: Near N-Terminus
KLH-conjugated linear peptide corresponding to human phospho PSD95 (Thr19) near the N-Terminus.
Application
Anti-phospho PSD95 Antibody (Thr19) is an antibody against phospho PSD95 (Thr19) for use in Western Blotting, Peptide Inhibition Assay, Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected phospho PSD95 (Thr19) in human cerebellum, human cerebral cortex, and human thalamus tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling
Physical form
Affinity
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in human fetal brain tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected phospho PSD95 (Thr19) in 10 µg of human fetal brain tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable (paraffin),inhibition assay: suitable (peptide),western blot: suitable |
| NCBI accession no. | NP_001122299 |
| UniProt accession no. | P78352 |
| shipped in | wet ice |
| target post-translational modification | phosphorylation (pThr19 ) |
| Gene Information | human ... DLG4(1742) |
