General description
RASSF1, also known as Ras association domain-containing protein 1, and encoded by the gene RASSF1/RDA32, is an important cell cycle and cell proliferation regulator protein. RASSF1 acts as a tumor suppressor and operates within the death receptor dependent apoptotic pathways. Additionally, RASSF1 inhibits proliferation at the G1/S boundary by regulating the accumulation of cyclin D1 protein. RASSF1 is localized to the cytoplasm and cytoskeleton depending upon cellular environment. RASSF1 localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. The protein also co-localizes with MDM2 in the nucleus. RASSF1 is expressed in multiple isoforms but in generally it is ubiquitously expressed but down regulated in cancer and tumor cells.
~39 kDa observed
Immunogen
GST-tagged recombinant protein corresponding to human RASSF1.
Application
Detect RASSF1 using this Anti-RASSF1 Antibody, clone 5C9.1 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:50-500 dilution from a representative lot detected RASSF1 in human kidney and human prostate cancer tissue.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected RASSF1 in 10 µg of human brain tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABE526
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 5C9.1, monoclonal |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_009113 |
| UniProt accession no. | Q9NS23 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... RASSF1(11186) |
