General description
Protein A purified rabbit polyclonal antibody. Recognizes the ~55 kDa (apparent MW) protein disulfide isomerase protein.
Immunogen
Bovine
bovine liver Protein Disulfide Isomerase
Application
Immunoblotting (1:1000, colorimetric)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (see comments)
Physical form
In PBS, 50% glycerol.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells, rat testis, mouse spleen
Other Notes
Cribb, A.E., et al. 1997. J. Pharmacol. Exp. Ther.282, 1064.
Freedman, R.B., and Hillson, D.A. 1980. In Enzymology of post-translational modification of proteins. (Freedman R.B. and Hawkin H.C. eds.) Academic, London p. 157.
This antibody will also work in immunocytochemistry, immunoprecipitation, and paraffin sections; the concentration must be determined for each sample analyzed. Variables associated with assay conditions will dictate the optimal working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | rabbit |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | liquid |
| contains | ≥0.9% sodium azide as preservative |
| species reactivity | guinea pig, Xenopus, sheep, bovine, rat, canine, hamster, porcine, mouse, monkey, human |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (Immunoblotting (1:1000, colorimetric) Immunocytochemistry Immunoprecipitation Paraffin Sections ) |
| isotype | IgG |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... PDIA2(64714) |
