General description
The integrin family of cell adhesion receptors consists of several membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented.
Platelet membrane glycoprotein Integrin beta3 or CD61 (GP IIIa) forms a Ca2+-dependent heterodimer complex with GP IIb. The GP IIb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets.
~ 86-87 kDa
Immunogen
Recombinant protein corresponding to mouse Integrin beta-3.
Application
Detect Integrin beta-3 (CD61) using this Anti-Integrin beta-3 (CD61) Antibody, clone HMb3-1 validated for use in FC, IH.
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Piali, 1995).
Biochem/physiol Actions
The antibody recognizes Integrin beta-3.
Physical form
Format: Purified
Analysis Note
Control
Mouse bone marrow-derived cells
Evaluated by Flow Cytometry on mouse bone marrow-derived cells.
Flow Cytometry Analysis: 0.1-1 µg/mL of the antibody detected Integrin beta-3 on mouse bone marrow-derived cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | hamster (Armenian) |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | HMb3-1, monoclonal |
| species reactivity | mouse |
| technique(s) | flow cytometry: suitable,immunohistochemistry: suitable |
| isotype | IgG |
| NCBI accession no. | NP_058060.2 |
| UniProt accession no. | O54890 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Itgb3(16416) |
