Immunogen
Murine L cells transfected with human alpha9.
Application
Detect Integrin α9β1 using this Anti-Integrin α9β1 Antibody, clone Y9A2, azide free validated for use in FC & IP.
Immunoprecipitation
FACS Analysis
Function blocking: 100% inhibition of alpha9beta1-mediated adhesion to the third fibronectin type III repeat in tenascin at <10 μg/mL.
Not effective for immunohistochemistry, immunocytochemistry or western blot.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins
Biochem/physiol Actions
Reactive with human and guinea pig alpha9beta1.
Physical form
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.
Protein A purified
Preparation Note
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Integrin alpha9beta1 positive tissues include basal keratinocytes, hepatocytes, muscle (smooth and skeletal), and airway epithelium.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | Y9A2, monoclonal |
| species reactivity | human, guinea pig |
| should not react with | rat |
| manufacturer/tradename | Chemicon® |
| technique(s) | flow cytometry: suitable,immunoprecipitation (IP): suitable |
| isotype | IgG1 |
| suitability | not suitable for Western blot,not suitable for immunohistochemistry |
| NCBI accession no. | NM_002207.2 |
| UniProt accession no. | Q13797 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ITGA9(3680) |
