General description
160 kDa Observed The translation of LDLR mRNA yields a 120 kDa protein that is post-translationally modified in the Golgi apparatus into the mature, 160 kDa LDLR. (Tolleshaug, H., et al. (1982). Cell 30:715–724.)
Low-Density Lipoprotein (LDL) Receptor is a mosaic protein that mediates the endocytosis of cholesterol-rich LDL. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). Mutations in LDLR are associated with familial hypercholesterolemia which is characterized by high levels of LDL in the blood.
Immunogen
Partially purified bovine adrenal LDL receptor
Application
Research Category
Metabolism
Research Sub Category
Lipid Metabolism & Weight Regulation
This Anti-LDLR Antibody, clone C7 is validated for use in WB for the detection of LDLR.
Biochem/physiol Actions
This antibody recognizes LDLR.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
ARPE-19 cell lysate
Evaluated by Western Blot in ARPE-19 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected LDLR on 10 µg of ARPE-19 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | C7, monoclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | bovine (based on 100% sequence homology) |
| technique(s) | western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_001160002 |
| UniProt accession no. | P01131 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... LDLR(3949) |
