General description
Anti-Green Fluorescent Protein (26-39), rabbit polyclonal, recognizes the ~28 kDa GFP and some GFP fusion proteins. It is validated for use in Western blotting and immunocytochemistry.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa GFP and some GFP fusion proteins.
Recognizes the ~28 kDa GFP and some GFP fusion proteins.
Immunogen
Aequora victoria
a synthetic peptide corresponding to amino acids 26-39 of Aequorea victoria wild-type green fluorescent protein
Application
Immunoblotting (1:2000)
Immunocytochemistry (1:200)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 1 mg/ml BSA.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Cubitt, A.B., et al. 1995. Trends Biochem. Sci.20, 448.
This sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. This antibody has been successfully used in immunoblotting and immunocytochemistry protocols to detect GFP and fusion proteins. It detects a ~28 kDa protein that corresponds to GFP in immunoblots of protein extract from cells transfected with, and overexpressing, GFP isoforms. Antibody should be titrated for optimal results in individual sytems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | rabbit |
| Quality Level | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| form | liquid |
| contains | ≤0.1% sodium azide as preservative |
| species reactivity | (GFP and some GFP fusions) |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (Immunoblotting (1:2000) Immunocytochemistry (1:200)) |
| isotype | IgG |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
